Biology (0610) Paper 6 Notes

IGCSE Biology (0610)
Paper 6 Alternative to practical

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(1) The humidity did not change during the investigation.
State two other variables that should be kept constant during the investigation.
Describe how each variable could be kept constant.
- temperature: keep in the same room/put into an environmental chamber
- idea of no air currents/wind/draughts: keep all windows and doors closed
- (sun) light (intensity): use a light source at a fixed source/same light source
- leaf surface area: use leaves of same size/surface area

(2) How would you improve the experiment [exercise and breathing rate environment]
- To improve experiment of exercise, increase exercise time, do different levels of intensity, cross out anomalies, controls
- Control: same intensity/time of exercise, temperature, clothing, diet of person
When describing about exercise, mention what heart does, like more oxygen is required so heart beats faster

(3) Making comparisons (e.g. seeds)
- When making comparisons, comment of shape/outline (is it smooth? Are there projections?), symmetry (regular/irregular),…

(4) Describe how you could safely test a sample (of any food) for reducing sugar
- Benedict’s reagent / solution / test;
- heat / boil; NOT warm/burn
- safety feature: goggles / water bath / tongs;
- correct colour change: blue to green / yellow / orange /red;

(5) A student carried out the emulsion test on a sample of yolk and it gave a positive result. State what the student would observe.
- cloudy / white solid / milky / white (emulsion);

(6) Describing graphs. Refer to these points:
- general increase from… to…
- are increases or decreases smooth
- figures with years/time/…
- general decrease from… to…
- peak/maximum at…

(7) Suggest why a sample of ten seedlings is better than a sample of only three seedlings
- (more) reliable/you are able identify anomalies 

(8) How could you carry out an experiment to determine whether temperature affects water uptake of shoots with leaves
Three of:
1 idea of at least three temperatures tested
2 suitable range of temperatures
3 control variable (eg light / wind / height or volume of water / humidity)
4 method of measuring rate of water uptake
5 Repeat for reliability

(9) [experiment on enzyme… and gas being released] Suggest how you could improve the method that they followed
- repeat and calculate mean
- exclude anomalies from mean calculation
- collect the gas and measure the volume

(10) The students were given a hypothesis that said
“the activity of catalase increases with increasing temperature”.
Describe an investigation to test this hypothesis.
- give a range of at least 4 temperatures;
- describe how temperature would be changed / water-bath;
- describe the use of a controlled equilibration time to reach temperature;
- control stated as: an inert cube / boiled cube or same volume of water as hydrogen peroxide;
- appropriate description of how volume of gas will be measured / bubbles counted;
- appropriate statement regarding time
- surface area of potato controlled
- another controlled variable stated, e.g. pH / same potato
- repeat and calculate mean

(11) A student criticised the results by saying that the loss in mass does not allow for a fair comparison between leaves. Suggest a more appropriate calculation and explain why it gives a fairer comparison.
- percentage change in mass = (change in mass ÷ starting mass) × 100
- different original mass would affect result/to take into account the starting mass

(12) [experiment with yeast] Example of description and explanation: describing solutions A and B
 (a) description
 1 number of bubbles in a minute increases with time 
 2 A released bubbles, more/faster (than B) 
 3 (colour change) from red to, yellow/yellow pink with time 
 4 A changed colour faster (than B) 
 5 A formed foam, more/ faster (than B
 6 suitable comparative data quote at a stated time

 (b) explanation
 7 releasing gas by respiration 
 8 gas released carbon dioxide 
 9 carbon dioxide is acidic 
 10 causes hydrogen carbonate indicator solution to change from red to yellow 
 11 A is respiring aerobically/B is respiring anaerobically 
 12 (the rate of) gas released in anaerobic respiration is slower

(13) Why was the solution covered with oil [experiment with yeast, glucose]
- to exclude air or oxygen

(14) Why was the temperature made warmer
- warm temperature speeds up enzyme/yeast activity

(15) How was the rate of respiration of yeast determined?
- bubble production/ colour change of hydrogen carbonate/ foam production 
- divided by time 
For rate, you need to write ‘divided by (unit) time’

(16) Describe a control experiment [for investigation with enzymes]
- idea of replacing the peroxide with water/ leaf with paper/using a boiled leaf 

- reference to keeping the rest of the experiment unchanged/describe the same experimental conditions 

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